感染流行性出血热病毒大鼠体液因素变化研究

Changes of humoral factors in rats infected with epidemic hemorrhagic fever viru

建立离乳大鼠腹腔感染流行性出血热病毒（ＥＨＦＶ）模型，应用荧光和放免技术，观察了大鼠体内多种神经递质及血管活性物质的变化。结果：感染组大鼠血浆中的儿茶酚胺（ＮＥ、ＤＡ）、５－羟色胺系统（５－ＨＴ、５－ＨＩＡＡ）、组胺（ＨＭ）和血栓素代谢产物（ＴＸＢ２）等较正常对照组显著增高，前列环素代谢产物（６－Ｋ－ｐＧＦ１ａ）则明显降低，后两者的比值增大。实验表明，ＥＨＦＶ所致的病毒血症引起机体应激和免疫病理反应，造成神经内分泌活动的加强及其代谢的紊乱。结果提示，在ＥＨＦ病程中，体液因素的变化有重要的病理生理学意义，纠正体液因素的紊乱是ＥＨＦ的一条重要的治疗原则。

A model of wealing rats infected with epidemic hemorrhagic fever virus(EHFV) by abdomen cavity was established,and fluorometry and radioimmunoassay were used to observe content change of neurotransmitter and blood vessel activation substance in normal and infected with EHFV rats. In rats infected with EHFV, the NA, DA, 5-HT, 5-HIAA, HM and TXA2 in plasma increased significantly, PGI2 decreased remarkedly, and the ratio of TXA2/PGI2 rised. The viremea caused by EHFV induced the stress and immunopathologic reaction. All such reactions caused neuroendorcine enhancement and metablism disturbance.It is suggested that the changes of humoral factors in EHF pathologic process played an important role in pathophisiology. To correct the disturbance of humoral factors was an important principle in treating EHF.