摘要
应用地高辛标记探针的原位杂交技术对25例口腔粘膜下纤维性变(OSF),5例口腔扁平苔藓(OLP),5例正常口腔粘膜(NOR)石蜡标本组织中转化生长因子β1(TGFβ1)mRNA进行了检测。结果显示:15例(15/25,60%)OSF组织中有TGFβ1mRNA表达,而5例OLP及5例NOR组织中均为阴性表达。8例初、早期OSF组织中,有7例(87.5%)TGFβ1mRNA表达呈阳性,而17例中、晚期OSF组织中,只有8例(47.0%)组织中TGFβ1mRNA表达呈阳性,两组间之异有显著性(P=0.0262,α=0.05)。本研究结果提示:转化生长因子β1在OSF的发病机理中可能起重要作用,转化生长因子β1可能是作为一中间介质参与OSF纤维化过程。
The transformin growth factorβ1(TGFβ1)mRNA in the paraffin-embeded tissues of 25 cases of Oral submucous fibrosis(OSF), 5 cases of oral lichen planus(OLP) and 5 cases of normal oral mucous(NOR) was determined by the in situ hybridization technique with a digox labeled probe. The result showed that there were expressions of TGFβ1 mRNA in the 15 OSF tissues(60%), while no positive results were obtained in the tissues of 5 OLP and 5 NOR. The authors divided the 25 OSF into two groups according to the course of the disease. 7 out of the 8 cases of early stage OSF(87.5%)had a positive result of TGFβ1 mRNA expression, out of the 17 cases of advanced stage OSF, however,8 cases(47.0%)showed positive. The difference of TGFβ1mRNA expression between the two groups was significant (p=0.0262 α=0.05). The study suggests that TGFβ1 may play an important role in the pathogenesis of OSF.The TGFβ1mRNA expression level is high during the early stage and declines gradually as the disease develops. It shows that TGFβ1may participates as a mediator in the fibrotic process of OSF.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1996年第1期20-22,共3页
West China Journal of Stomatology
关键词
口腔粘膜
纤维性变
转化生长因子Β
RRNA
oral submucous fibrosis transforming growth factorβ_1 in situ hybridization