口腔粘膜下纤维性变组织中转化生长因子β_1mRNA表达水平

The mRNA Expression of Transforming Growth Factorβ_1 in Oral Submucous Fibrosis Tissues──In Situ Hybridization Study

应用地高辛标记探针的原位杂交技术对２５例口腔粘膜下纤维性变（ＯＳＦ），５例口腔扁平苔藓（ＯＬＰ），５例正常口腔粘膜（ＮＯＲ）石蜡标本组织中转化生长因子β１（ＴＧＦβ１）ｍＲＮＡ进行了检测。结果显示：１５例（１５／２５，６０％）ＯＳＦ组织中有ＴＧＦβ１ｍＲＮＡ表达，而５例ＯＬＰ及５例ＮＯＲ组织中均为阴性表达。８例初、早期ＯＳＦ组织中，有７例（８７．５％）ＴＧＦβ１ｍＲＮＡ表达呈阳性，而１７例中、晚期ＯＳＦ组织中，只有８例（４７．０％）组织中ＴＧＦβ１ｍＲＮＡ表达呈阳性，两组间之异有显著性（Ｐ＝０．０２６２，α＝０．０５）。本研究结果提示：转化生长因子β１在ＯＳＦ的发病机理中可能起重要作用，转化生长因子β１可能是作为一中间介质参与ＯＳＦ纤维化过程。

The transformin growth factorβ1(TGFβ1)mRNA in the paraffin-embeded tissues of 25 cases of Oral submucous fibrosis(OSF), 5 cases of oral lichen planus(OLP) and 5 cases of normal oral mucous(NOR) was determined by the in situ hybridization technique with a digox labeled probe. The result showed that there were expressions of TGFβ1 mRNA in the 15 OSF tissues(60%), while no positive results were obtained in the tissues of 5 OLP and 5 NOR. The authors divided the 25 OSF into two groups according to the course of the disease. 7 out of the 8 cases of early stage OSF(87.5%)had a positive result of TGFβ1 mRNA expression, out of the 17 cases of advanced stage OSF, however,8 cases(47.0%)showed positive. The difference of TGFβ1mRNA expression between the two groups was significant (p=0.0262 α=0.05). The study suggests that TGFβ1 may play an important role in the pathogenesis of OSF.The TGFβ1mRNA expression level is high during the early stage and declines gradually as the disease develops. It shows that TGFβ1may participates as a mediator in the fibrotic process of OSF.