人类口腔粘膜癌前损害发生发展过程中CDNK2基因作用的研究：Ⅰ.

The Research of the Roles of CDKN_2 Gene in the Carcinogenesis of Human Oral Mucosa(Ⅰ).A Preminelary Observation of P16 Expression Level by Immunohistochemical Method

新近发现，ＣＤＫＮ_２基因是第一个直接参与细胞周期调控的抑癌基因，但ＣＤＫＮ_２基因及其产物Ｐ１６蛋白在口腔粘膜癌前损害发生、发展过程中的作用及变化尚未见公开报导。本研究采用ＬＳＡＢ免疫组织化学染色技术研究发现：上皮单纯增生、上皮异常增生及无转移浸润性鳞状细胞癌中均有Ｐ１６蛋白表达，其阳性反应既可见于胞核，也可见于胞浆；随着细胞恶性程度的增高其阳性反应强度及阳性细胞数量呈递增趋势，且阳性反应的异质性明显增加。至转移性浸润性鳞状细胞癌阶段，Ｐ１６蛋白的阳性反应强度稍弱于前几个阶段的结果；转移浸润癌的原发灶及其转移淋巴结中的肿瘤上皮Ｐ１６蛋白的阳性反应都仅限于胞浆。

CDKN_2 is a newly-reported tumor suppressor gene that controls cell cycle in direct pathway. But the report on the roles of CDKN_2 gene and its product, P16 protein, in the process of oral carcinogenesis is scarce, The results of this research showed immunohistochemically that the tissues of hyperkeratosis, premalignant lesions and invasive but nonmetastaticsquamous cell carcinomas had P16 expression. The positive staining appeared in cell nuclei and cytoplasms. And the positive degree and the heterogenecity of positive reactions trended to increase gradually with the development of cell maligancy. On the other hand,the positive degree in the tissues from patients with metastatic invasive squamous cell carcinomas was slightly lower than that of the other tissues and the positive stainingonly located in the cytoplasms of the primary lesions and their metastatic lymph nodes. It indicates that the P16 protein plays some roles in the carcinogenesis of human oral mucosa.