摘要
白介素12和18在宿主保护性免疫中起着非常重要的作用,它可以诱导γ-干扰素的产生。众所周知,在结核感染早期,γ-干扰素的诱导是产生保护性 T 细胞的基础。在体外和体内试验中,活的卡介苗都可以诱导出较高的γ-干扰素水平,而热杀死的卡介苗不能。在本研究中,我们调查了小鼠 PEC 细胞分别经活卡介苗和热杀死卡介苗刺激后,所产生的 IL-12p40,IL-18和γ-干扰素的水平。通过对卡介苗培养过滤液诱导细胞因子能力调查,来探索为什么活的卡介苗可以诱导较高的γ-干扰素,而热杀死卡介苗却不能。研究发现,活的卡介苗可以激活巨噬细胞,诱导出 IL-12p40和 IL-18,而死的卡介苗却不能。活的卡介苗的培养过滤液可以诱导出 IL-12p40。而热杀死卡介苗培养过滤液却不能产生 IL-12p40。研究表明,活的卡介苗分泌到培养上清液中的分子质量为130kD 的蛋白类物质是细胞因子的诱导产生的关键因素。
IL- 12 and IL- 18 play a very important role in host protective immunity.They can induce the expression IFN -γ.It is well known that IFN -γ produced at the initial stage of BCG infection is essential for the generation of protective T cells and viable BCG inducing protective T cells exhibits a higher IFN -γ inducing activity than killed BCG in both vivo and vitro. In our study, we have examined the levels of IL - 12p40 and IL- 18 as well as IFN-γ produced by mice peritoneal exudate eell（PEC）in response to viable or killed BCG.The culture filtrate was examined to find out why viable but not killed bacteria induce a strong IFN-γ production. Viable BCG could activate maemphage to produce both IL- 12p40 and IL- 18. Culture filtrate also showed IL- 121340 inducing activity.In contrast, killed BCG did not induce IL- 12p40 and IL - 18 production. Because the euhure filtrate of killed BCG did not show the activity, it appeared that molecular weight 130kD protein secreted by viable BCG to the euhure supematant contributes to the production of eytokine.
出处
《医学研究通讯》
2005年第10期15-17,共3页
Bulletin of Medical Research
