尿NAG测定用于严重烧伤后肾功能损害的监测

N-Aety l - Beta-Glucosaminidase in Urine of Patients with Severe Burns

采用荧光基质４－甲基伞型酮－β－Ｄ－基葡萄糖苷荧光法，动态观察了５９例严重烧伤病人尿ＮＡＧ酶活性。结果表明，严重烧伤后尿ＮＡＧ酶活性显著升高，持续在较高水平，伤后４１天仍显著高于正常对照。尿ＮＡＧ与ＢＵＮ、Ｃｒ呈显著正相关，与Ｃｃｒ呈显著负相关。尿ＮＡＧ活性增加与烧伤面积和烧伤深度密切相关。肾功能衰竭组尿ＮＡＧ显著升高。动态观察尿ＮＡＧ活性可了解病变的轻重、病程归转及影响因素，为临床判定严重烧伤病人肾损伤提供一个敏感有用的非创伤性方法。

N-Acetyl-Beta-g lucosaminidase(NAG)has been studied with the enzyme substrate(methy-lumbelliferone-N- Acetyl-beta-glucosaminidase)fluo-rescence assay in the urine of 59 patients with severe-ly burned. The results demonstratedthat total uri-nary NAG excretion increased significantly postburn,and this increments sustained up to 27 days then de-clined,but still h igher than normal value even at 41PBD.The elevated level of urine NAG activity corre-lated positively w ith extent of burn injury as well asthe severity of postburn renal dan1age,Thesechanges of the urine NAG activity correlated posi-tively with the blood UN,Cr and occured earlier thanthose of the routine paran1eters such as BUN,Cr.Therefore,total urine NAG excretion can be em-ployed to detect the early damage of proximal tubulepostburn。