生物转化对甲基苯甲酸合成对苯二甲酸的研究

Biotransformation of p-toluic acid into terephthalic acid by Comamonas testosterone DSM6577

在单加氧酶和脱氢酶系的作用下,睾丸酮丛毛单胞菌DSM6577以对甲基苯甲酸(p-TOL)为唯一碳源,生物催化生成对苯二甲酸(PTA),并对其细胞生长、底物代谢和产物生成过程进行了研究。结果表明,底物在8 h内即可完全代谢,但检测到产物的生成需要更长的时间。

Comamonas testosterone strain DSM6577 was shown to be capable of oxidizing p-toluic acid （p-TOL） as sole souce of carbon into terephthalic acid through sequential reactions catalyzed by a series of enzymes including monooxygenase and dehydrogenase. The strain＇s growth, p-toluic acid＇s degradation and terephthalic acid production were studied respectively, p-Toluic acid was completely degraded within 8 hours but it took a longer time when the terephthalic acid produced could be determined.