鼻咽癌中p53蛋白积聚的生物学特征和临床意义

Biological characteristics and clinical implications of p53 protein accumulation in nasopharyngeal carcinoma

收集３４７例鼻咽癌组织，全部或部分进行了ＣＭ－１、ｐＡｂ２４０、ｐＡｂ１８０１和ｐＡｂ４２１四种ｐ５３蛋白抗体以及ＰＣＮＡ和Ｋｉ６７抗体的染色。综合分析染色结果并结合组织学特征，临床分期以及预后等资料，得出以下结论：①鼻咽癌中ｐ５３蛋白的积聚率为４４．３％，积聚的ｐ５３蛋白其抗原性不完全一样；②ｐ５３积聚的癌细胞具有增殖活性较强和侵袭性较弱的特点；③ｐ５３蛋白积聚的百分率在无颈淋巴结转移的原发瘤中比有颈淋巴结转移者高；在早期原发瘤中比晚期原发瘤中高；在放疗后存活５年以上者中比放疗后１．５年内死亡者中高。

CM-1 polyconal antibody was uesd for detecting the p53 protein in 347 nasopharyngeal carcinomas,154 out of 347 NPCs had been found to be p53 protein accumulation and its percentage being 44.3%. Besides CM-1,pAb 240, pAb1801 and pAb421 were simutaneously used for detecting the p53 protein in 40 NPC cryosections,and the results illustrated that the antigenic of the accumulated p53 protein was not all the same. The consecutive tissus sections were also stained by PCNA and Ki67. Cooparative observation showing that majority of CM-1 positive cells were PCNA or Ki67 positive. In addition, the percentage of p53 positive cells in Regaud growth pattern (57.0%)was higher than iri Schminke growth pattern(9.2%).The above results revealed that the positive tumor cells had their own biological characteristics, i. e. more proliferous activity and less invasive behavior.Our results also showed that the percentage of p53 protein accumulation in primary tumours of advanced NPC patients with more than 5-year survivals after radiotherapy was higher than that with distant metastasis and death immediately followed after radiotherapy.