鼻咽癌克隆株裸鼠移植瘤自发性转移及群体增殖能力的研究

STUDY OF SPONOTANEOUS METASTASIS AND POPULATION PROLIFERATION ABILITY OF TRANSPLANTABLE SUBCLONE CELLS OF NASOPHARYNGEAL CARCONOMAS IN NUDE MICE

将鼻咽癌克隆细胞株ＣＮＥ－２Ｚ－Ｈ５、Ｆ１及Ｈ５移植瘤之淋巴结及肺转移灶组织，移植入裸鼠皮下，观察其自发性转移；同时研究了转移组和非转移组移植瘤之间，不同生长时间瘤重、体积倍增时间及增殖细胞核抗原表达的差异。结果显示：各类移植瘤的转移能力及途径均不同；在一定生长时间内，转移组移植瘤重大于非转移组（Ｐ＜０．０５）．体积倍增时间较非转移组短（Ｐ＜０．０５），增殖细胞核抗原表达较非转移组强。提示：淋巴结及肺转移灶组织中可能存在高转移特性、且为不同转移途径的异质性转移亚群；肿瘤细胞转移和增殖能力存在一定的关系。

The nasopharyngeal cell line CNE-2Z-H5,F1 and lymph node of H5 transplantable tu-mor and lung metastatic tissues were xenografted subcutaneously to node mice,The characteristics of spono-taneous metastases were observed.Furthermore,we have studied the differences in the doubling time,thetumor weight,and the expression of proliferating cell nuclear antigen(PCNA)between metastatic groupand nonmetastatic group at different time,The results showed thet the metastatic ability and pathways inall kinds of transplantable tumors were different.The tumor weight of metastatic group increased fasterthan nonmetastatic group(P<0.05),the doubling time of metastatic group was shorter than nonmetastaticgroup(P<0.05),and the expression of PCNA in metastatic group was stronger than that in nonmetastaticgroup within some times.These results suggested that there may be some heterogeneous subpopulation ofdifferent metastatic ability and pathways in lymph node and lung metastatic foci.There are some relation-ships between the metastasis and proliferation ability.