p53基因对鼻咽癌细胞株CNE－3裸鼠致瘤抑制作用的研究

STUDIES ON THE ROLE OF SUPPRESSION OF P53 ON HUMAN NASOPHARYNGEAL CARCINOMA CELL GROWTH IN NUDE MICE

分别将野生型和突变型ｐ５３基因导入鼻咽癌的体外培养细胞系ＣＮＥ－３．裸鼠致瘤试验表明，导入野生型ｐ５３基因的细胞系，肿瘤生长速度明显低于对照细胞系及导入突变型ｐ５３基因的细胞系；导入突变型ｐ５３基因的细胞系肿瘤生长速度最快；导入野生型ｐ５３基因的细胞系肿瘤生长速度最慢，而且肿瘤出现时间也较导入突变型ｐ５３的细胞及对照细胞晚１～２周。这说明野生型ｐ５３基因能有效地抑制肿瘤的生长，而实变型ｐ５３基因则可以促进肿瘤生长。这是首次有关ｐ５３基因对鼻咽癌细胞作用的报道。这一研究更进一步说明了ｐ５３基因的功能，对于鼻咽癌发生机理的探讨以及肿瘤的防治具有重要意义。

Wild-type and mutant human P53 genes were transfected into the nasopharyngeal carcino-m3(NPC)cell line CNE-3.Tumorigenicity in nude mice showed that the tumor resulting from the cellstransfected with the wild-type P53 gene grew more slowly and was smaller than that from control CNE-3cells.In contrast,the tumor from the cells transfected with the mutant P53 gene grew faster than that pro-duced by cells transfected with the wild-type P53 gene and that producd by control CNE-3 cells.The re-sults demonstrate that the wild-type P53 gene could inhibit the NPC cell growth in nude mice and the mu-tant P53 gene could enhance the NPC cell growth in nude mice.The P53 gene may also play an importantrole in the pathogenesis of NPC.