单剂量黄曲霉毒素B_1致大鼠肝癌作用的短期实验模型

SHORT TERM EXPERIMENTAL ANIMAL MODEL OF HEPATOCEARCINOGENESIS INDUCED BY SINGLE DOSE of AFLATOXIN B1 IN RATS

本文报告单剂量黄曲霉毒素Ｂ１（ＡＦＢ１）致大鼠肝癌作用短期实验模型的研究．６周龄、雄性Ｗｉｓｔａｒ大鼠，经腹腔一次性注射不同剂量（０、０．５０、０．７５、１．００和１．５０ｍｇ／ｋｇ体重）的ＡＦＢ１，作为启动剂。两周后，饲以含０．０１５％的２－乙酰氨基芴（２－ＡＡＦ）饲料４周。实验第三周末，施行肝大部分切除术（ＰＨ）。所有动物于实验第６周处死，取肝组织作Ｇａｍｍａ－谷氨酰转肽酶（ＧＧＴ）化学染色，然后对ＧＧＴ阳性肝细胞灶进行定量分析。结果显示单剂量ＡＦＢ１加２－ＡＡＦ及ＰＨ选择促进程序能诱发癌前肝细胞酶变灶，而ＡＦＢ１的启动作用有明显的剂量反应关系。

A short term experiment for the induction of hepatocellullar precancerous lesions by singleof aflatoxin B1(AFB1) was studied.Five groups of 6-week-old inbred wistar Rats were given byI.P,withdifferent doses of AFB1(0,0.50,0.75,1.00,1.25 and 1.50mg/kg bw).Two groups of rats was served ascontrols given by DMSO only or without pretreatment.After two weeks,the animals were given by 0.015%2AAF-diet for four weeks.At the third week of experiment,partial hepatectomy was conducted.The ani-mals were sacrificed at sixth week.The liver tissues were processed for Gamma-glutamyl-transpeptidase(GGT)stain.The GGT postive hepotocytic hyperplastic foci were induced and revealed dose dependent ef-fect.