利用缺体回交法选育小黑麦异代换系的研究

A research on nullisomic backcross method for breeding alien substitution lines

以4D缺体为母本,黑麦为父本进行杂交,授粉后16～18d进行幼胚培养,培养的试管苗用0.05％秋水仙素处理,加倍后的植株再用缺体回交1～2次,就可获得小黑麦异代换系。在第二次回交的F_1(B_1F_1)代里,单体代换植株占18.5％;双单体代换占24％,这两种类型经自交后都获得了4D/4R异代换系,有的异代换系已直接用于生产。

The embryoes of F, hybrids between 4D nullisomic wheat, as female,and rye,as male, were cultured after fertilizing 16 to 18 days,and the FI plantlets were doubled under the treatment of 0.05% eolchicine-The doubled plantlets were then backcrossed with nullisomics one to two times,thus wheat-rye alien substitution lines could be obtained.There were about 18-5% of monosomic substitution plants and about 24-0% of double monosomic substitution in the BiF, Aline substitution lines 4D/4R were produced by their selfing.Now some of the aline substitution lines have been applied directly to production.