氯酚类物质测定新方法及其与人血清白蛋白的相互作用

New Methods for the Determination of Chlorophenols and the Interaction Between Them with Human Serum Albumin

以2,4-DCP为代表,分别考察了溶液酸度、试剂用量、反应温度和反应时间等因素对该体系荧光猝灭强度的影响;由Stern-Volmer方程求出2,4-DCP与HSA的猝灭常数(Kq)及反应热力学参数.新建方法线性范围为0.75～6.50 μg/10 mL,检出限为2.26×10-10g/mL(以2,4-DCP计).Kq=3.713×1013L/(mol·s).该方法结合C18柱固相萃取技术,用于水中CPs的测定,相对标准偏差小于2.91%,加标回收率为96.44%～105.94%,结果满意.2,4-DCP对HSA内源荧光的猝灭以静态猝灭为主.

Objective： Establishing new method for the determination of chlorophenols （CPs）. Studying the association reaction mechanism of CPs with HSA. Methods： The affecting factors on the strength of fluorescence quenching including acidity, dosage, temperature and reaction time etc were investigated. According to Stern-Volmer equation, the quenching constant of the reaction and the basic thermodynamic parameters of the blinding process were obtained. Results： The detection limit was 2. 26 × 10^-10 g/mL and the linearity range were 0. 75～6. 50μg/10 mL. The fluorescence quenching constant Kq was 3. 713 × 10^13 L/ （mol·s）. Conclusion： When the proposed method was used to determine CPs （2,4-DCP） in water, combined with solid phase extractor （SPE）, the satisfactory results were obtained and the relative standard deviation was less than 2. 91% and the recovery range was 96. 44%～105. 94%. Fluorescence quenching mechanism of 2,4-DCP with HSA was a static quenching procedure.