摘要
使用Inertsil SIL-100硅胶柱,以正己烷等为流动相的正相液相色谱方法分析乳粉中的β-胡萝卜素收到了较好的效果.该方法的检出限、重现性、回收率和线性范围分别为:0.220μg/mL,1. 88%,90.2%～95.1%和2.280～456.000μg/mL.关于采用正相液相色谱法分析β-胡萝卜素的方法未见报到.该方法具有快速,灵敏度高的特点,保留时间仅为3 min左右,并大大地缩短了分析时间,提高了效率.
A new HPLC method for analysis of β-carotene in milk powder was established. The retention time of β-carotene was about 3.5 min, phase were （n-hexane＋cyelohexane＋iso-propyl-alcohol=0.46＋0.46＋0.08）＋chloroform = 99＋1. Linear ranges of determination is 2.280～456.000μg/mL, and the limits of determination of the analysis method is 0. 220μg/mL. Recovery founded in the milk powder sample is in the range of 90.2%～95.1%.
出处
《分析测试技术与仪器》
CAS
2005年第4期307-310,共4页
Analysis and Testing Technology and Instruments
