摘要
为提高枯草杆菌工程菌外源基因的表达水平，探索了蔗糖诱导型枯草杆菌工程菌表达系统DB1342（pSCl8—66）的表达条件，筛选到蔗糖诱导型枯草杆菌工程菌最佳表达条件为：在枯草杆菌对数生长前期加蔗糖进行诱导表达，蔗糖的诱导剂量控制在2％～4％，诱导表达时间为20～24h．
To enhance the expression efficiency of heterologous gene in B. subtilis, The conditions of the high efficient expression of B. subtilis engineered strain DB1342（pSC18 -66） induced by sucrose was studied. The results showed that sucrose was added at the front middle stage of growth and induced for 20 - 24 hours with the concentration of 2% - 4% were suitable for B. subtilis to express heterologous gene.
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2005年第6期13-16,共4页
Journal of Henan University of Technology：Natural Science Edition
基金
河南工业大学引进人才专项基金(150162)
关键词
枯草杆菌
蔗糖诱导
高效表达
B. subtilis
sucrose induced
high efficient expression
