摘要
通过对二个品种樱桃砧木的叶片和叶柄的诱导和分化研究,认为两种砧木的叶柄再生能力都强于叶圆盘,BA作用强于KT,NAA优于IBA。综合平衡后可总结出一种诱导和分化芽的通用配方熏即:M S+BA 1～2mg/L+KT1～2mg/L+NAA 0.5mg/L+IBA 0.5mg/L。再生芽的频率最高可达42%～67%。1/2M S培养基附加生根粉(ABT)2mg/L,黑暗处理3d后,生根率和移栽成活率能达到91.8%～93.8%。
Through the studies of the induction and differentiation of leaves and petioles of two varieties of cherry rootstock ,we got the conclusion that the differentiating ability of petioles is more efficient than the leaf-discs ,BA does better than KT,NAA is more effective than IBA.From all these factors considered, we get the a formula used both for shoot induction and subculture ： MS＋BA 1 -2 mg/L＋KT 1-2 mg/L＋NAA 0.5 mg/L＋ IBA 0.5 mg/L.The highest frequency of shoot induction reaches 42%-67%.In the medium of 1/2MS with ABT 2 mg/L,the rooting rate can reach 91.8% and the survive rate can reach 93.8%.
出处
《北方果树》
2006年第1期5-7,共3页
Northern Fruits
关键词
吉塞拉樱桃砧木
组织培养
快繁
正交设计
Cherry rootstock of Gisela
Plant tissueculture
Rapid propagation
Orthogonal design
