非小细胞肺癌组织中AKT2、CyclinD1、MMP-9表达及其与临床病理因素的关系

Expression of AKT2, Cyclin D1, and MMP-9 and Their Correlations to Clinicopathologic Features of Non-small Cell Lung Cancer

背景与目的:AKT2是一种丝氨酸/苏氨酸激酶,已被确定为癌基因,在多种肿瘤组织中都存在AKT2的异常表达和活化,AKT2与肿瘤的增殖和侵袭转移密切相关。本研究旨在探讨AKT2、CyclinD1、MMP-9在非小细胞肺癌(non-smallcelllungcancer,NSCLC)中的表达及与临床病理因素的关系。方法:应用免疫组化的方法检测68例NSCLC组织、38例相应的癌旁组织和14例非肿瘤性肺组织标本中AKT2蛋白、CyclinD1、MMP-9的表达,采用!2检验分析临床病理因素与上述指标的相关性。结果:AKT2、CyclinD1、MMP-9在NSCLC组织中阳性率分别为91.2%、76.5%、72.1%,显著高于癌旁及非肿瘤性肺组织中阳性率(3.8%、0%、13.5%)(P<0.05)。AKT2的表达与患者年龄、性别、肿瘤类型及分化程度、TNM分期无关(P>0.05),与淋巴结转移有关(P<0.05)。CyclinD1、MMP-9表达与淋巴结转移、鳞癌的分化程度有关(P<0.05),MMP-9还与TNM分期有关(P<0.05)。AKT2的表达与CyclinD1、MMP-9呈正相关。结论:AKT2、CyclinD1、MMP-9均与肺癌的发展有关,CyclinD1、MMP-9的高表达可能与AKT2的调节有关。

BACKGROUND ＆ OBJECTIVE： AKT2, a serine/threonine kinase, is confirmed to be an oncogene. Abnormal expression and activation of AKT2 is observed in many kinds of tumor tissues. AKT2 is associated with the proliferation and invasion of cancers. This study was designed to investigate the expression of AKT2, Cyclin D1, and matrix metalloproteinase- 9 （MMP-9） in human non-small cell lung cancer （NSCLC） tissue and their correlations to clinicopathologic features of NSCLC. METHODS. The expression of AKT2, Cyclin D1, and MMP-9 in 68 specimens of NSCLC, 38 specimens of corresponding adjacent tissues, and 14 specimens of nocancerous lung tissue were assessed by immunohistochemistry; their correlations to clinicopathologic factors were analyzed using Chi-square test. RESULTS. The positive rates of AKT2, Cyclin D1, and MMP-9 were significantly higher in NSCLC than in adjacent tissues and no-cancerous lung tissues （91.2% vs. 3.8%, 76.5% vs. 0%, 72.1% vs. 13.5%, P〈0.05）. The expression of AKT2 wasn＇t correlated to age, sex, histologic subtype and differentiation, and TNM stage of NSCLC patients （P〉0.05）, but was correlated to lymph node metastasis （P〈0.05）. The expression of Cyclin D1 and MMP-9 was correlated to lymph node squamous cell carcinoma （P 〈0.05）; correlated to TNM stage. The expression the expression of Cyclin D1 and MMP-9 metastasis and the expression differentiation of of MMP-9 was of AKT2 was positively correlated to （P〈0.05）. CONCLUSIONS. AKT2, Cyclin D1, and MMP-9 is related to development of lung cancer. The overexpression of Cyclin D1 and MMP-9 may relate to AKT2 regulation.