宫颈癌中p27^(Kip1)基因的表达与体外药物敏感性的关系

Relationship between the expression of p27^(Kip1) and chemosensitivity of cervical carcinoma in vitro

背景与目的:p27K ip1基因是调控细胞周期并抑制细胞分裂的重要基因。它与诱导肿瘤细胞的凋亡和化疗药物的耐药也有关系。本文旨在探讨宫颈癌组织中p27K ip1基因的表达与体外药敏的关系。方法:采用MTT法检测了部分化疗药物在46例宫颈癌组织中的抑制率,并采用免疫组织化学方法检测了癌组织中p27K ip1基因的表达。结果:宫颈癌组织中p27K ip1表达的阳性率为15.2%(7/46)。顺铂(DDP)、表柔比星(E-ADM)、替尼泊苷(VM26)、紫杉醇(Taxol)、吉西他滨(Gem zar)、氟尿嘧啶(5-FU)和BLM的平均抑制率分别为47.6%、38.0%、42.7%、27.4%、23.0%、26.3%和24.8%。DDP、VM26和Taxol在p27K ip1表达阴性的宫颈癌标本中的抑制率显著低于阳性标本(P<0.05),而在其他药物中差异无显著性。结论:p27K ip1的表达可作为判断肿瘤对化疗药物的敏感性的指标,指导临床用药。

Background and Purpose： p27^Kip1 is an important gene that inhibits cellular division and regulates cell cycle, it promotes cell apoptosis, and is also related to chemotherapeutic drug tolerance. This paper is to study the relationship between the expression of p27^Kip1 and chemosensitivity of cervical carcinoma in vitro. Methods： We used MTT assay to detect the inhibitory rate of some chemotherapeutic drugs in 46 samples of cervical carcinoma in vitro. The expression level of p27^Kip1 was detected by immunohistochemistry method. Results： The positive rate of p27^Kip1 of cervical carcinoma was 15. 2% （7/46）. The average inhibitory rates of DDP, E-ADM, VM26, Taxol, Gemzar, 5-Fu and BLM were 47.6%, 38.0%, 42.7%, 27.4%, 23.0%, 26.3% and 24.8%, respectively. The inhibitory rates of DDP, VM26, Taxol in the cervical carcinoma with p27^Kip1 negative were significantly lower than those with p27^Kip1 positive samples （P 〈0.05）. However there were no significant differences in the other therapeutic drugs. Conclusions： The expression of p27^Kip1 can be used to detect the chemosensitivity in cervical carcinoma and direct drug administration.