AN EXPERIMENTAL STUDY ON TRANSPLANTATION OF FROZEN CANINE PHALANGEAL JOINT ALLOGRAFTS INCORPORATED WITH AUTOGENIC BONE MARROW

目的 探讨经过多重钻孔并复合自体骨髓的冷冻异体犬足趾关节移植的疗效。方法 24只健康成年杂交犬，制备双侧后肢第2足趾近侧趾间关节1．5cm缺损模型，共48侧，随机分成A、B、C3组（n=16）。分别采用新鲜自体趾间关节（A组）、经钻孔并复合自体新鲜骨髓的冷冻异体趾间关节（B组）及单纯冷冻异体趾间关节（C组）修复缺损。于术后1、3、6和12个月分别行X线摄片和组织病理学检查，了解移植骨关节的成活情况。结果 根据移植关节术后X线片和组织学改变，犬趾骨关节移植后的病变可分为轻度、中度和重度变性3级。A组移植骨关节3～12个月始终表现为轻度变性；B组移植骨关节1～6个月为轻度变性，骨孔中央软骨内成骨现象明显，12个月部分移植骨关节为中度变性；C组从1个月出现移植骨关节中度变性，3个月移植骨关节重度变性。结论 冷冻异体犬足趾关节内多重钻孔并复合自体新鲜骨髓．能有效延缓异体骨关节的早、中期变性，单纯冷冻异体骨关节移植后早期可能发生严重变性。

Objective To investigate the result of the transplantation of frozen canine phalangeal joint allografts perforated and incorporated with autogenic bone marrow. Methods A proximal interphalangeal joint defect of 1.5 cm was prepared at bilateral sides of twenty-four adult healthy out bred dogs. Three different types of allografts were applied to repair the defects fresh autogenic phalangeal joints （group A, n： 16）, frozen phalangeal joint allografts perforated and incorporated with fresh autogenic hone marrow（group B, n= 16）, and frozen phalangeal joint allografts （group C, n=16）. Radiographic and histological study were used to evaluate the survival of transplanted joints. The observation was done 1, 3, 6 and 12 months after operation respectively. Results Based on the radiographic and histological changes of the transplanted joints, the osteoarthropathy of transplanted canine phalangeal joints could be divided into 3 degrees： mild degeneration, moderate degeneration and severe degeneration. Mild degeneration was observed in group A from 3 to 12 months. Mild degeneration was also found in group B from 1 to 6 months, and the endochondral ossification was obvious within the drilled bony holes. However, some joints in group B underwent moderate degeneration 12 months after operation. Group C joints in the first month had moderate degeneration, which progressed to severe degeneration 3 months after operation. Conclusion Transplantation of frozen canine phalangeal joint allografts perforated and incorporated with autogenic bone marrow can effectively delay the degeneration of transplanted osteoarticular allografts at the early and middle stage.