摘要
目的检测粘蛋白MUC5AC在翼状胬肉中的表达。方法应用免疫组化PABC法,分别对19例翼状胬肉标本和5例正常结膜组织中的MUC5AC表达进行检测。比较2组间的染色级别差异。结果5例正常结膜中,2例MUC5AC蛋白染色阳性(+),2例为阳性(),1例为阴性;19例翼状胬肉组织中,1例MUC5AC蛋白染色阴性,3例为阳性(+),7例为(),8例为()。MUC5AC蛋白定位于细胞浆,胞核无着色。阳性部位主要在上皮中的杯状细胞。翼状胬肉组的染色级别高于对照组(P<0.05)。结论MUC5AC在翼状胬肉中表达增加。
Objective To detect the expression of MUCSAC in pterygium. Methods The immunohtstochemical methods PABC was performed in study of expression of MUCSAC in 19 pterygial specinlens and 5 normal conjunctiva. The grades of stain were compared between groups. Results Among 5 normal conjunctiva, there were 2 positive（ ＋ ）,2 positive（＋＋） and 1 negative; while among 19 pterygial specimens, there were 1 negative,3 positive（ ＋ ）,7 positive（＋＋） and 8 positive（＋＋＋）. The protein of MUCSAC is located in the cytoplasm and mainly in the epithelial goblet cells. Pterygium had higher grade of stain than nornml conjunctiva（P 〈 0.05）. Conclusion The expression of MUCSAC in pterygium is increased compared with that in conjunctiva.
出处
《眼科新进展》
CAS
2006年第1期32-33,共2页
Recent Advances in Ophthalmology
