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激活T细胞负性共刺激信号对大鼠同种异体肾移植存活的影响 被引量：5

Influence of PDL1Ig gene transfection on the survival of renal allografts.
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摘要 目的观察激活负性共刺激信号程序性死亡因子及配体(PD1-PDL1)对延长移植肾存活的效能。方法以PDL1Ig基因重组腺病毒为载体,将PDL1Ig基因转入BN大鼠供肾,以Lewis大鼠为受体,行同种肾移植为转染组,并以未转染BN供肾移植给Lewis受体为对照组;观察移植肾存活时间和术后肾功能变化。结果转染组移植肾存活(36±7.3)d,较对照组移植肾存活时间(8.6±1.2)d明显延长;移植组术后血清肌酐较同期对照组明显为低。结论PDL1基因局部转染可明显延长移植肾存活时间。 Objective To observe the efficacy of the hypothesis that stimulating programmed death 1 （PD-1 ）-triggered negative signals on the survival of renal allografts. Methods The kidneys of BN mrs were transfeeted with PDLI Ig gene-recombined adenovirus. The transfeeted kidneys were transplanted to Lewis rats（ transfeetion group）. BN→Lewis kidney transplantation with non-transplanted kidneys served as controls.The allograft survival time and the allograft function between the two groups were compared.Results The allograft survival time of transfection group was loger than that of controls significantly （36 ± 7.3d vs 8.6 ± 1.2d, P 〈 0.05） .The serum creatinine of transfection group at 8th postoperative days were lower than that of controls at 8th postoperative day. Conclusion Local transfection of PDLIIg gene can prolong the survival time of renal allograft significantly.
作者 冯嘉瑜 张艮甫 黄赤兵 王平贤 范明齐
机构地区 第三军医大学新桥医院
出处 《四川医学》 CAS 2006年第1期4-5,共2页 Sichuan Medical Journal
基金 国家自然科学基金资助项目(30200264) 国家自然科学基金重点项目(3993430-2)
关键词 程序性死亡因子1 基因治疗 肾脏移植 共刺激信号 PDLI Ig gene therapy kidney transplantation co-stimulatory signals
分类号 R699.2 [医药卫生—泌尿科学]
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参考文献11

  • 1Latchman Y,Wood CR, Chernova T, et al. PD-L2 is a second ligand for PD- 1 and inhibits T cell activation [J]. Nat Immunol, 2001,2 (3) : 261 -268.
  • 2Gao W, Demirci G, Strom TB, et al. Stimulating PD-1-negative signals concurrent with blocking CD154 co-stimulation induces long-term islet allograft survival [J] .Transplantation,2003,76(6) :994 - 999.
  • 3Kanai T, Totsuka T, Uraushihara K, et al. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation [J]. J Immunol, 2003,171 (8) :4156 - 4163.
  • 4Mazanet MM and Hughes CC. B7-H1 is expressed by human endothelial calls and suppresses T cell cytokine synthesis [J]. J Immunol, 2002, 169(7) :3581 - 3588.
  • 5Carter L, Fouser LA, Jussif J, et al. PD- 1 : PD- L inhibitory pathway affects both CD4( + ) and CD8( + ) T cells and is overcome by IL-2 [J] .Eur J Immunol,2002,32(3) :634 - 643.
  • 6黄赤兵,方玉华,吴军,罗高兴,曹川,马兵.应用新技术建立大鼠肾移植模型[J].中华器官移植杂志,2001,22(2):114-114. 被引量：39
  • 7冯嘉瑜,张艮甫,黄赤兵,张寅生,贺伟峰,吴军.改良的大鼠原位肾移植模型的建立[J].第三军医大学学报,2004,26(24):2264-2265. 被引量：12
  • 8Racusen LC, Solez K, Colvin RB, et al. The Banff 97 working classification of renal allograft pathology [J] .Kidney Int, 1999,55(2) :713 - 723.
  • 9Fechner H, Haack A, Wang H, et al. Expression of coxsackie adenovirus receptor and alphav-integrin does not correlate with adenovector targeting in vivo indication anatomical vector barriers [J] . Gene Ther, 1999,6(9) :1520 - 1535.
  • 10冯嘉瑜,张艮甫,王平贤,范明齐,贺伟峰.CD154胞外区基因局部修饰对大鼠移植肾存活的影响[J].第三军医大学学报,2004,26(12):1068-1070. 被引量：5

二级参考文献23

  • 1[1]Yamada And A, Sayegh M H. The CD154-CD40 costimulatory pathway in transplantation[J]. Transplantation, 2002, 73(1 Suppl): S36-39.
  • 2[2]Xu H, Tadaki D K, Elster E A, et al. Humanized anti-CD154 antibody therapy for the treatment of allograft rejection in nonhuman primates[J]. Transplantation, 2002, 74(7): 940-943.
  • 3[3]Yin D, Ma L, Varghese A, et al. Intact active bone transplantation synergizes with anti-CD40 ligand therapy to induce B cell tolerance[J]. J Immunol, 2002, 168(10): 5352-5358.
  • 4[7]Racusen L C, Solez K, Colvin R B, et al. The Banff 97 working classification of renal allograft pathology[J]. Kidney Int, 1999, 55(2): 713-723.
  • 5[8]Fechner H, Haack A, Wang H, et al. Expression of coxsackie adenovirus receptor and alphav-integrin does not correlate with adenovector targeting in vivo indicating anatomical vector barriers[J]. Gene Ther, 1999, 6(9): 1520-1535.
  • 6[10]Pearson T C, Trambley J, Odom K, et al. Anti-CD40 therapy extends renal allograft survival in rhesus macaques[J]. Transplantation, 2002, 74(7): 933-940.
  • 7[11]Xu H, Elster E A, Blair P J, et al. Effects of combined treatment with CD25- and CD154-specific monoclonal antibodies in non-human primate allotransplantation[J]. Am J Transplant, 2003, 3 (11) : 1350-1354.
  • 8Densem CG, Mutlak ASM,Pravica V, et al. A novel polymorphism of the gene encoding furin, a TGF-β1 activator, and the influence on cardiac allograft vasculopathy formation[J]. Transplant Immunology, 2004,37 (7): 697～ 702
  • 9Citterio F, Pozzetto U, Romagnoli J, et al. Plasma levels of transforming growth factor-β1 in renal transplant recipients receiving different immunosuppressive regimens[J] .Transplantation Proceedings,2004,36(3) :698 ～ 699
  • 10Morales JM, Andres A, Rengel M, et al. Influence of cyclosporin, tacrolimus and rapamycin on renal function and arterial hypertension after renal transplantation[ J]. Nephrol Dial Transplant,2001,16( 1 ): 121 ～ 124

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四川医学
2006年 第1期

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