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抑肽酶对内皮细胞β-链蛋白保护作用的实验研究

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摘要 目的体外观察人肿瘤坏因子α(TNFα)对内皮细胞细胞连接蛋白β-链蛋白的影响及抑肽酶的保护作用,探讨抑肽酶抑制体外循环术后系统性炎性反应的机制。方法将体外培养的人脐静脉内皮细胞随机分为空白组,TNFα组,抑肽酶+TNFα组。采用免疫细胞化学法观察人脐静脉内皮细胞连接蛋白β-链蛋白(-βcatenin)的表达。用细胞培养小室测定中性粒细胞跨迁单层融合的人脐静脉内皮细胞的迁移率。结果250KIU/ml抑肽酶可显著减少-βcatenin的丢失(P<0.05);250KIU/ml抑肽酶对中性粒细胞跨迁活化的单层人脐静脉内皮细胞有显著影响(P<0.05)。结论抑肽酶对内皮细胞细胞连接具有保护作用;抑肽酶可能通过保护内皮细胞细胞连接来抑制体外循环术后系统性炎性反应。
作者 陈朝辉 徐昆
机构地区 川北医学院附属医院 重庆医科大学附属第二医院
出处 《四川医学》 CAS 2006年第1期15-17,共3页 Sichuan Medical Journal
关键词 抑肽酶 Β-CATENIN 体外循环 系统性炎性反应
分类号 R654.1 [医药卫生—外科学]
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四川医学
2006年 第1期

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