红花对家兔肠系膜微血管运动作用机理的在体研究

Effect of Carthamustinctorius Linne on Rabbit Mesentery vasomotion Induced by Noradrenaline In Vivo

目的:观察红花(CTL)对家兔肠系膜微血管运动的影响,并探讨一氧化氮(NO)在其中的作用,以进一步明确红花活血化瘀的作用机理。方法:取健康雄性大耳白家兔20只,以乌拉坦腹腔注射行基础麻醉,氯醛酮耳缘静脉注射维持麻醉,气管插管置家兔于自发呼吸状态,同步记录呼吸、血压。在活体情况下运用血管运动诱导法诱导出血管运动后分别观察静注CTL后血管运动的变化及应用NOS抑制剂L-NMMA对其的影响,将结果摄录到监视器上,用暂停功能测量。结果:静注CTL后可使由去甲肾上腺素(NA)诱导出的血管运动被抑制于舒张状态,应用L-NMMA后CTL的作用未受影响。结论:CTL抑制血管运动的作用机制不是通过内源性NO介导的。

Objective： Observing CTL effect on mesentery vasomotion and exploring the No action in the effect, to assure the CTL mechamism in activiting blood to resolve blood stasis. Methods： 20 Albino rabbits weighting 2.0 - 2.5 kg were anesthetized with urethane （0.75g/kg） and alpha-chloralose （30mg/kg） intravenously. The rabbits were ventilated with room air via tracheotomy tube. The arterial pressure and breath were monitored with a pressure transducer attached to a polyethylene canuula in the right common carotid artery by four channel polygraph, the intestinal loop was mounted on the stage of an inverted microscope and bathed in balanced Kreb-Ringer solution maintained at 37.5℃ .The inside diameter of arterioles were measured manually on a TV monitor using a TV camera mounted on the microscope. Changes of BP and mlcrocirculation were observed after injection of NA intravenously. CTL （200mg/ml/kg） and L-NMMA （10mg/ml） were administered via a cannula in the left carotid artery after topical application of NA （0.8μg） conducted in the measured field. Results ： Spontaneous vasomotion can be induced in the measured field after NA topically used. CTL can inhibit vasomotion in vasoconstriction stage, L-NMMA does not influence the effect of CTL on vasomotion. Conclusions： CTL＇s inhibition on vasomotion does not gain through endogenous NO.