蜡蚧轮枝菌昆明菌株的生物学特性研究(一)——温度对菌落生长、产孢量和分生孢子萌发率的影响

A Study of Biological Character of Verticillium lecanii KM9803 Strain Isolated from Kunming

蜡蚧轮枝菌昆明菌株KM9803菌丝生长、分生孢子产生和萌发的温度范围是5～35℃，适温范围是15～30℃，最适温度范同是23～28℃，最适温度25℃。当温度20～28℃时，14h分生孢子萌发率为98．6％～100％。在奁彼克培养基（CzA）上，20～28℃15d平均产孢量范围是1．3×10^9～4．9×10^9孢子／皿，16h分生孢子萌发率93，67％～100％。25℃最适于产孢。

Strain KM9803 was an aphid-derived isolate of Verticillium lecanii （Zimm） Viegas from Brassica oleracea var capitata L. in greenhouse of Yunnan Agricultural University in 1998. Temperature of Mycelium growth, conidiospore production and germination of this isolate occurred between 5 - 35 ℃ ;The suitabler temperature were between 15 -30 ℃;The suitablest were between 23 -28 ℃ and the optimal was 25 ℃ for it, growth, spore production and germination. The germination rate of conidiospore within 14 h was 98.6% - 100% in 20 - 28 ℃. On CzA media, sporulation yield was ranging from 1.3 × 10^9 to 4.9 × 10^9 spores/dish in 15 days in 20 -28 ℃ ,while 25 ℃ as optimal.