开胃丸的薄层鉴别与含量测定研究

Studies of TLC and Quantitative Determination of Kai Wei Wan

目的制定开胃丸的质量控制方法。方法对白术、厚朴、陈皮与枳实进行了薄层色谱鉴别,用高效液相法测定了制剂中厚朴酚与和厚朴酚的含量。结果通过方法学考察,厚朴酚的进样量在0.243～1.944μg,和厚朴酚的进样量在0.15925～1.274μg范围内,呈良好的线性关系。厚朴酚的平均回收率为100.97%(n=5),RSD为0.84%;和厚朴酚的平均回收率为99.48%(n=5),RSD为0.84%。结论方法简便、准确、重现性好、精密度高。可用于控制开胃丸的质量。

Aim To establish a method for the quality control of Kai Wei Wan. Method Rhizoma Atractylodis Macrocephalae, Cortex Magnoliae Officinalis, Pericarpium Citri Reticulatae and Fructus Aurantii Immaturus were identifed by TLC. The content of magnolol and honokiol in Kai Wei Wan was determinated by HPLC. Results The methodological study showed that a good linear correlation existed in the range of 0.243 - 1.944μg（ r = 0.999 99） of magnolol and 0. 15925 1.274μg （ r = 0.999 95）of honokiol applied respectively .The average recovery rate （ n = 5） was 100.97% for magnolol （ RSD was 0.84% ） and 99.48% for honokiol （ RSD was 0.84% ）. Conclusion This method is simple, accurate and with good repetivity and high precision. The method can be used for quality control of Kai Wei Wan.