摘要
目的应用聚合酶链反应(PCR)检测胃癌血清中的肿瘤相关抗原。方法常规操作9个步骤共30个循环,每一循环包括DNA变性,引物退火及链的延伸。结果 36例胃癌血清呈阳性者29例(803.%),30例对照组血清呈阳性者2例(6%),两者对比差异显著(P<0.01)。结论 PCR技术在胃癌血清学诊断的应用是迄今速度快,特异性强,敏感性高的检测方法。
AIMS To detect the serum gastric cancer associated antigen by using polymerase chain reaction (PCR). METHODS Thirty-six endoscopically and pathologically proved gastric cancer patients (age 46-76 averaging 62.5±5.3 years; males 24, females 12) and 30 non-gastric cancer associated antigen was detected by using PCR with 9 operational procedures, altogether 30 circules, each including degeneration of DNA, annealing of primer and extension of chain. RESULTS Serum gastric cancer associated antigen was positive in 29 out of 36 patients with gastric cancer (80.5%), and only in 2(6.0%) out of 30 controls. The difference was very significant (P<0.01). CONCLUSIONS PCR method in serologic diagnosis of gastric cancer is till now a useful one with great spead, strong specificity and high sensitivity, because tumour genes or DNA can be amplifed more than millionfold.
关键词
胃肿瘤
肿瘤抗原
聚合酶链反应
stomach neoplasms tumor antigens immunoenzyme technics
