小檗碱促进大鼠胃溃疡愈合的实验研究

Effects of berberine on experiment gastric ulcer

目的观察小檗碱对大鼠胃醋酸性溃疡的愈合作用。方法采用改良 Okabei 法,用80%醋酸诱发大鼠慢性胃溃疡模型,随机分2组,对照组(n=6)以生理盐水0.5 ml/d 灌胃,实验组(n=6)给小檗碱0.1 g/(kg·d)灌胃,连续10 d。11 d 后将鼠断颈处死,剖腹取胃,测量溃疡的横径和竖径,求出平均半径,计算溃疡面积(mm^2)。取大鼠(n=8)实验前禁食24 h(自由饮水),实验时先用戊巴比妥(50 mg/kg,ip)麻醉,用灌流方法每过30 min收集1次胃引流液,第1小时收集的为基础胃液,1 h 后经十二指肠注入小檗碱(0.1 g/kg),每隔30min 收集1次,共4次,最后将所收集的胃液分别用0.1 mol/L NaOH 滴定,测量酸度和计算酸排出量。结果溃疡面积由对照组的0.37±0.08 mm^2减少到实验组的0.11±0.02 mm^2(P<0.05)。小檗碱抑制大鼠基础胃酸的分泌,对照组的30 min总酸排出量为0.11±0.05(mmol/30 min),给药后30,60,90及120 min 分别是0.05±0.01,0.04±0.01,0.03±0.01及0.02±0.01(mmol/30min),给药后60,90及120 min 胃酸总排出量明显减少(P<0.01)。结论本实验发现小檗碱对大鼠慢性胃溃疡有促进愈合作用,机制为小檗碱抑制胃酸作用有关。

AIMS To study the effects of berberine on gastric ulcer caused by acetic-acid in rats. METHODS The model of gastric ulcer caused by 80% aceti- cacid in 12 rats,according to improved Okabei methods, treated with berberine 0.1 g(kg·d)for 10 days.Then ulcer area was measured pathologically.The rats(n=8),with- out giving any food for 24 h(only drinking water freely), were narcotized with pentobarbital(50 mg/kg ip).The drainage gastric juice were collected every 30 min.After that,berberine(0.1 g/Kg)poured through duodenum, and the gastric were collected juice every 30,4 times in all. Finally the gastric juice was titrated with 0.1 mol/L NaOH,measuring the acidity and the discharge. RESULTS Berberine could promote the healing of chronic gastric ulcer induced by acetic-acid.Ten days administra- tion of berberine reduced the size of ulcer significantly(P <0.05).The ulcer area in the control group was 0.24±0. 08 mm,in experimental group 0.11±0.02 mm.The reults indicated that berberine inhibited acid sceretion.After 60, 90 and 120 min of administration of berberine the acid se- cretion was 0.04±0.01,0.03±0.01 and 0.02±0.01 mmol/30 min(P<0.01).