罗汉果黄酮的活血化瘀药理作用研究

Study on the Pharmacological Function of Stimulate Circulation to End Stasis of Flavone from Momordica grosvenori

为了观察罗汉果黄酮的活血化瘀药理作用,采用水提和树脂吸附方法提取罗汉果黄酮,采用尾静脉注射胶原蛋白和肾上腺素造成小鼠体内血栓,测定药物对小鼠脑血栓的保护率;用腺苷-5-二磷酸钠盐(ADP)诱导大鼠血小板聚集,计算药物对血小板聚集的抑制率;腹腔注射75%蛋黄乳液造成实验性高血脂症,测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白含量;采用毛细玻璃管法测定小鼠凝血时间,并进行了的急性毒性试验。结果表明,罗汉果黄酮对血栓形成有一定的保护作用;能抑制ADP诱导大鼠血小板聚集;明显降低高胆固醇血症小鼠的TC和TG含量,提高HDL-C的水平;以及延长小鼠的凝血时间。说明罗汉果黄酮具有一定的抗血栓形成、抗血小板聚集、降血脂、抗凝血等活血化瘀药理作用。急性毒性试验结果表明,罗汉果黄酮对小鼠的最大给药量为60.85g/kg。

To evaluate the pharmacological function of stimulate circulation to end stasis effects,of flavone from Mornordica grosvenori. Extracted flavone from M. grosvenori by water and method of absorption resin; injection of collagen and adrenalin through vein of tail of mice causing thrombus, and detected protection-rate from thrombus in brain by flavone;induced aggregation of platelet in rats by ADP method, calculating inhibition of flavone on aggregation of platelet;hyperlipidemia in mice was induced by intraperitoneal injection of 75% solution of egg yolk,and concentrations of total cholesterin （TC）, triglyceride （TG） and high density lipoprotein （HDL） was detected ; detected coagulation time （CT）in mice by capillary tube method; acute toxicity assay was also performed. Result：flavone from M. grosvenori could inhibit formation of thrombus and aggregation of platelet induced by ADP;could decrease the concentration of TC and TG in mice with hyperlipidemia,and increase the level of HDL-C in mice, while prolonging CT. Flavone from M. grosvenori have functions of antithrombogenicity, inhibition of thrombus formation, aggregation of platelet and could decrease the level of TG,TC and HDL,and acute toxicity assay suggests that maximum dose of administration to mice is 60.85g/kg.