MCMV感染对小鼠海马[Ca^(2+)]i及线粒体膜电位的影响

Effect of [Ca^(2+)]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytome-galovirus infected mice

目的:观察鼠巨细胞病毒(MCMV)感染对小鼠海马细胞内游离Ca2+浓度(犤Ca2+犦i)及线粒体膜电位的影响。方法:将BALB/C乳鼠同母鼠随机分为实验对照组(24只)和病毒组(35只),感染后56d应用荧光分光光度计和流式细胞仪分别检测两组小鼠海马的犤Ca2+犦i及线粒体膜电位。结果:病毒感染组小鼠海马犤Ca2+犦i增高及线粒体膜电位明显下降,与实验对照组比较差异有显著性(P值均<0.01)。结论:巨细胞病毒感染所引起的小鼠海马犤Ca2+犦i及线粒体膜电位的改变可能是其导致神经系统功能紊乱的机制之一。

Objective To explore effect of [Ca^2＋]i and neuronal mitochondria transmembrane potentials in hippocampus of mice cytomegalovirus（MCMV） infected mice. Methods Newborn mice and femal mice of BALB/C were randomly divided into two groups： the virus inoculated group and the control group. After 56 days, single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the intracellular free calcium level [Ca^2＋]i in hippocampus were measured by means of fluorospectro-photometer and flow cytometry （FCM）. Results Compared with the control group, the mitochondria transmembrane potentials was decreased （P 〈 0. 01） and the intracellular free calcium level [Ca^2＋]i was increased （P 〈 0.01） in inoculated group. Conclusion The dysfunction of [Ca^2＋]i and mitochondria transmembrane potentials in hippocampus may play an important role in the CMV infected brain.