鸡A-FABP基因多态性分析及其与脂肪性状的相关研究

Study of Single Nucleotide Polymorphism of A-FABP Gene and Its Association with Fatness Traits in Chicken

以北京油鸡为试验材料,对A-FABP基因进行单核苷酸多态性(SNPs)检测和基因型与性状的关联分析。方差分析结果表明,不同基因型间腹脂率、皮脂厚、肌内脂肪含量差异极显著(P<0.01),体重在不同基因型间差异不显著(P>0.05)。由此推测,A-FABP可能为影响鸡脂肪代谢的主效基因或与主效基因相连锁。

In this experiment, Beijing-You chicken was used to detect single nucleotide polymorphisms （SNPs） in A-FBAP gene and to study the correlation between its genotype and the trait of fat accumulation, The first exon of the gene was amplified by one pair of primers, and SNPs were detected by the technique of single strand conformation polymorphism （SSCP） and finally confirmed by sequencing. Results of analysis of variance showed that a significant difference existed among abdominal fat percentage, subcutaneous fat thickness and intramuscular fat contents in breast musculature with different genotypes （ P〈O. 01）. It implied that A-FABP gene could be a major effector gene or could be linked to gene（s） which significantly affect fat metabolism in chicken.