摘要
曲茎遗传材料NG94-156与遗传背景不同的3个正常茎品种杂交,获得1个F2群体和2个重组自交家系(F7∶8)。后代分离分析的结果表明,NG94-156的曲茎性状受两对隐性重叠基因控制。利用4个亲本和1个重组自交家系筛选260个RAPD随机引物,其中有1个引物S-506扩增出的多态性条带有较好的重复性。经过连锁分析,RAPD标记S-5061600与控制曲茎的基因的遗传距离为6.94 cM。
Three crosses between NG94-156 （brachytic stem） and three varieties （normal stem） were made, and F2 segregative population and two recombined inbred line populations（F7： 8 ） were obtained. Genetic analysis indicated that the brachytic stem of NG94-156 was controlled by two duplicate recessive genes. In searching for RAPD marker linked to the genes controlling brachytic stem, 260 RAPD primers were applied to screen four parents of three combinations and RIL. Polymorphic bands revealed by the primer S-506 exhibited the best repeatability among all primers. Linkage analysis indicated the genetic distance between S-5061600 and brachytic stem gene was 6.94 cM.
出处
《遗传》
CAS
CSCD
北大核心
2006年第1期50-56,共7页
Hereditas（Beijing)
基金
国家973资助项目(编号:2004CB7206)
国家自然科学重大项目(编号:30490250)~~
