基于PMT的激光共聚焦生物芯片微弱荧光检测

Laser Confocal Biochip Scanner Based on PMT

介绍了激光共聚焦扫描仪的成像原理以及PMT的特性和控制原理,在此基础上详细分析了FPGA通过DAC动态控制PMT增益的微弱荧光信号检测方法,以适应不同的荧光强度,FPGA同时控制16位高速AD转换,得到高精度图像数据。实验结果表明,系统具有高精度和高分辨率的特点,可探测到0.1个荧光分子/μm2。

The imaging principle of the laser confocal biochip scanner, the property and control principle of PMT are introduced. And on the base of the above discussion, the weak fluorescent detection that the gain of the PMT controlled by FPGA through digital to analog converter （DAC） dynamically to adapt different fluorescent intensity is analyzed. The high speed 16-bit analog to digital converter （AD） is also controlled by FPGA, and high precision image data is obtained. The experiments prove that it can detect 0.1 fluorophor/μm^2, the system is high in precision and resolution.