放线菌素D对持续感染乙脑病毒复制的影响

Effect of actinomycin D on the virus replication of persistent encephalitis B virus infection

目的：研究放线菌素D对持续感染乙脑病毒增殖活性的影响。方法：将乙脑病毒感染人肝癌细胞株KN73，采用标准胰蛋白酶消化技术进行细胞传代，建立持续感染系，加入不同浓度的放线菌素D（0～2．0mg·L^-1），24h后收集细胞内病毒，应用空斑形成实验测定病毒含量，对比用药前后持续感染病毒量的变化。结果：用浓度为0．1，0．2，0．5，1．0，2．0mg·L^-1的放线菌素D处理感染细胞后，细胞内病毒量分别是对照组的1．89，3．33，4．34，4．52，4．85倍。结论：放线菌素D可使持续感染的乙脑病毒复制增加。

OBJECTIVE To observe the effect of actinomycin D on persistent infection of encephalitis B virus. METHODS Persistent infection model was established by using the standard strains of encephalitis B virus and KN73 cells. Cells were subeultured using standard trypsinization techniques. Different concentration of actinomycin D （ 0 - 2.0 mg·L ^-1 ） were added to persistently infected cells and cell-associated viruses were collected after 24 h. Virus titers were examined by plaque methods. RESULTS At the concentrations of 0.1 mg· L^-1 and 2.0 mg· L^-1 actinomycin D, virus yields were 1.89 and 4.85 times of that of control group respectively. CONCLUSION Actinomycin D can stimulate encephalitis B virus replication.