高效液相色谱法快速测定人血浆中盐酸伪麻黄碱的血药浓度

Rapid determination of pseudoephedrine hydrochloride in human plasma by HPLC

目的：用高效液相色谱法测定盐酸伪麻黄碱（PSE）的血药浓度。方法：色谱柱为Diamonsil C18（4．6mm×200mm，5μm），流动相为0．5％硫酸胺-甲醇（80：20），检测波长210nm，流速1．0mL·min^-1。结果：标准曲线的线性范围为3．125～20mg·L^-1（r=0．9993，n=7），低、中、高3种浓度的日内和日间RSD分别为4．31％，2．30％，2．07％（n=5）和5．83％，3．54％，4．16％（n=5），相对回收率分别为94．17％，101．22％，97．03％。结论：本方法简便、快速、准确、专一性好，可用于盐酸伪麻黄碱的血药浓度测定。

OBJECTIVE To determine the plasma drug concentration of PSE in rats with RP-HPLLC method. METHODS Diamonsil C18 （4.6 mm × 250 mm, 5 μm） was used and the mobile phase consisted of 0.5% ammonium sulfate-methanol （80： 20） at the flow rate of 1,0 mL· min^-1. The UV detection wave length was 210 nm. RESULTS The linear range was 3. 125 -200 mg·L^-1 （r = 0. 999 3, n = 7） and the limit of detection for PSE was 0.02 mg·L^-1. The precision of the intra-day and inter-day of three different concentration solutions were 4.31% ,2.30% ,2.07% （ n = 5 ） and 5.83% ,3.54% ,4.16% （ n = 5） respectively, and the extration recovery was 94.17%, 101.22% ,97.03% respectively. CONCLUSION The method is simple, rapid, sensitive, and reliable, and it is suitable for the determing of drug concentration of PSE in human plasma in clinical use .