反相高效液相色谱法测定人血浆中阿立哌唑的浓度

Determination of aripiprazole in plasma by RP-HPLC with UV detection

目的：建立测定人血浆中阿立哌唑浓度的HPLC法。方法：以Diamonsil^TMC18反相柱（250mm×4.6mm，5μm）为色谱柱，流动相为甲醇-0．03mol·L^-1醋酸铵（90：10），流速为0．8mL·min^-1，检测波长257nm，以乙醚为提取剂。结果：阿立哌唑的高（500μg·L^-1）、中（100μg·L^-1）、低（20μg·L^-1）3个浓度的提取回收率分别为93．51％，93．23％，96．51％，日内、日间RSD均小于6％；分析方法的最低定量限为5μg·L^-1。线性范围为5-500μg·L^-1，回归方程为C=376．24F-5．48，r=0．9997（n=9）。结论：该方法灵敏、准确、简单、快速，可用于临床血药浓度监测和药动学研究。

OBJECTIVE To develop an HPLC method for the quantitative determination of aripiprazole in plasma. METHODS Aripiprazole was extracted with diethyl ether. The residues were analyzed with a reverse phase HPLC system Diamonsil^TM C18 column （ 4.6 mm × 250 mm, 5 μm） with the mobile phase consisted of methanol-0.03 mol·L^-1 NH4 AC （ 90：10 ）. The detection wavelength was at 257 nm . RESULTS The average recoveries for aripiprazole were 93.51% , 93.23% and 96.51% , respectively. The within-day and between-day precision of variation was less than 6%. The calibration curves for aripiprazole had good linearity , r = 0. 999 7 （ n = 9）, over the range of 5-500 μg·L^-1. The limits of quantitation for aripiprazole was 5 μg·L ^-1. CONCLUSION The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of aripiprazole in plasma and its phamacokinetic studies.