摘要
目的:建立测定人血浆中阿立哌唑浓度的HPLC法。方法:以Diamonsil^TMC18反相柱(250mm×4.6mm,5μm)为色谱柱,流动相为甲醇-0.03mol·L^-1醋酸铵(90:10),流速为0.8mL·min^-1,检测波长257nm,以乙醚为提取剂。结果:阿立哌唑的高(500μg·L^-1)、中(100μg·L^-1)、低(20μg·L^-1)3个浓度的提取回收率分别为93.51%,93.23%,96.51%,日内、日间RSD均小于6%;分析方法的最低定量限为5μg·L^-1。线性范围为5-500μg·L^-1,回归方程为C=376.24F-5.48,r=0.9997(n=9)。结论:该方法灵敏、准确、简单、快速,可用于临床血药浓度监测和药动学研究。
OBJECTIVE To develop an HPLC method for the quantitative determination of aripiprazole in plasma. METHODS Aripiprazole was extracted with diethyl ether. The residues were analyzed with a reverse phase HPLC system Diamonsil^TM C18 column ( 4.6 mm × 250 mm, 5 μm) with the mobile phase consisted of methanol-0.03 mol·L^-1 NH4 AC ( 90:10 ). The detection wavelength was at 257 nm . RESULTS The average recoveries for aripiprazole were 93.51% , 93.23% and 96.51% , respectively. The within-day and between-day precision of variation was less than 6%. The calibration curves for aripiprazole had good linearity , r = 0. 999 7 ( n = 9), over the range of 5-500 μg·L^-1. The limits of quantitation for aripiprazole was 5 μg·L ^-1. CONCLUSION The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of aripiprazole in plasma and its phamacokinetic studies.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第1期50-52,共3页
Chinese Journal of Hospital Pharmacy
基金
广东省社会发展领域科技计划项目(编号:53060)