2型糖尿病并脑梗死患者eNOS基因内含子4上a/b基因多态性的初步研究

Research about the a/b in intron 4 polymorphism of endothelial nitric oxide synthase (eNOS) gene in type 2 diabetes with cerebral infarction

目的探讨内皮细胞性一氧化氮合酶(eNOS)基因内含子4上a/b基因的多态性与单纯2型糖尿病(NIDDM)及糖尿病并脑梗死(cerebral infarction,C I)的关系。方法应用聚合酶链反应(PCR)技术,检测89例NIDDM合并C I病人的基因型,并与对照组比较。结果NIDDM合并C I组eNOS基因ab基因型的频率(26.97%)明显高于对照组(12.5%)及NIDDM组(12.68%),a等位基因的频率(13.48%)也明显高于对照组(6.25%)及NIDDM组(6.34%),均有显著性差异(P<0.05)。结论eNOS基因ab基因型与NIDDM合并C I有相关性,等位基因a可能是NIDDM合并C I的危险因素。

Objective To investigate association between the a/b in intron 4 polymorphism of endothelial nitric oxide synthase （ eNOS} gene and type 2 diabetes with cerebral infarction（ CI）. Methods The genotype of 89 patients with type 2 disbetes with CI and control group and type 2 diabetes group was measured by polymerase chain reaction （ PCR）. Results The ab genotype distibution frequency of eNOS gene in diabetes group with CI is higher than that in control group （26.97% vs12.5%） and type 2 diabetes （ 12. 68%）. The a allele frequency of eNOS gene of diabetes group with CI is significantly higher than that in control group（ 13.48% vs6. 25%） ,and significantly higher than that in type 2 diabetes （6.34% ） , too. Statistical differences between diabetes group with CI and control group are significant （ P 〈 0. 05）, and between diabetes group with CI and diabetes group （ P 〈 0. 05 ）. Conclusion There is association between ab genotype of eNOS and diabetes with CI. The eNOS a allele may be a risk factor for diabetes with CI.