摘要
Objective: To study the 4977-bp deletion of mitochondiral DNA in lung cancer, adjacent normal tissue and health lung and its significance in the development of cancer. Methods:Thirty-seven matched lung cancer/adjacent histologically normal and 20 “true” normal lung tissue samples from patients without lung cancer were analyzed by long PCR technique. Results: Mitochondrial DNA 4977-bp deletion was detected in 54.1% (20/37) of lung cancers, 59.5% (22/37) of adjacent normal and 30.0% (6/30) of “true” normal lung tissues. The correlation of 4977-bp deletion with age and smoking factors was present in our data. Conclusion: Mitochondrial DNA 4977-bp deletion is not specific to lung cancer and unlikely to play an important role in carcinogenesis, and may only reflect the environmental and genetic influences during tumor progression.
Objective： To study the 4977-bp deletion of mitochondiral DNA in lung cancer, adjacent normal tissue and health lung and its significance in the development of cancer. Methods： Thirty-seven matched lung cancer/adjacent histologically normal and 20 ＂true＂ normal lung tissue samples from patients without lung cancer were analyzed by long PCR technique. Results： Mitochondrial DNA 4977-bp deletion was detected in 54.1% （20/37） of lung cancers, 59.5% （22/37） of adjacent normal and 30.0% （6/30） of ＂true＂ normal lung tissues. The correlation of 4977-bp deletion with age and smoking factors was present in our data. Conclusion.. Mitochondrial DNA 4977 bp deletion is not specific to lung cancer and unlikely to play an important role in carcinogenesis, and may only reflect the environmental and genetic influences during tumor progression.
基金
SupportedbyDevelopmentalProgrammingItemofNa-tionalKeystoneBasicResearch(G2000016106)andNatureScienceFoundationofChongqing(2005).
