组织芯片的有效性研究

A validation study on tissue microarrays

目的检测组织芯片的有效性.方法收集 70 例乳腺癌病例,制成直径为 2 mm的组织芯片,进行雌激素受体(ER)和孕激素受体(PR)免疫组织化学染色,同时与普通切片的免疫组化染色结果相对比,并进行统计学分析.结果组织芯片与普通切片的ER、PR染色积分(0～7 分)显著相关,ER、PR 的表达(阳性/阴性)一致率分别达 96.97%、93.93%.比较组织芯片与普通切片的 ER、PR 表达,两者之间无统计学差异(P＞0.05),即组织芯片的结果与普通切片的结果是一致的.结论采用规范的组织芯片制作程序,2 mm 直径的组织芯片在进行免疫组化染色时能够代表普通切片.该技术是一种可靠的、有代表性的或有效的高通量组织分子分析工具.

Objective To evaluate the validation of tissue microarrays. Methods Tissue microanays with 2 mm in diameter tissue core were constructed from seventy cases of breast cancer.The tissue microanays were stained for oestrogen receptor （ER） and progesterone receptor （PR） using immunohistochemistry.At the same time the stains on the tissue microarrays were compared with that from the full tissue sections. Results A highly significant association was observed between the staining scores （0 - 7） obtained from the full tissue sections and from the tissue microarrays. Concordance for the receptor status （positive/negative） of the whole section and the tissue core were 96.67% for ER, 93.93 % for PR. The discordance between full section and tissue microarrays was studied using Two - Related - Samples tests and the result had no statistical significance （ P 〉 0.05） . Conclusion Tissue micruarays with 2 mm in diameter tissue core can represent full tissue section on inanunohistochemistry study, if the tissue microarrays are constructed as standard method. We believe tissue microarray is a reliable and high - throughout tool for research.