斑节对虾杆状病毒包涵体研究

Study on occlusion body of Penaeus monodon baculovirus

利用显微图像分析技术,对斑节对虾肝胰腺正常细胞胞核和斑节对虾杆状病毒(MBV)感染细胞胞核横切面的直径、周长和面积进行了测量分析。结果表明:正常细胞胞核的直径为3.47±0.30μm、周长为13.03±1.36μm、面积为10.87±1.78μm2;MBV感染细胞胞核的直径为3.81±0.79μm、周长为14.00±2.87μm、面积为13.52±5.37μm2;两者的直径、周长和面积均存在极显著的差异(P<0.01)。对3种类型的包涵体进行了测量,其中1类包涵体最大,2类包涵体次之,3类包涵体最小,并以1类包涵体占大多数。

The diameters, perimeters and areas of normal cell nucleus and cell nucleus infected with Penaeus monodon baculovirus（MBV） in hepatopancreas epithelial cells of Penaeus monodon were studied by micro-imaging analytical technique. The results show that the mean diameter is 3.47±0.30μm, the mean perimeter is 13.03±1.36μm and the mean area is 10.87±1.78μm^2 in normal cell nucleus, while the mean diameter is 3.81±0.79μm, the mean perimeter is 14.00±2.87μm and the mean the is 13.52±5.37μm^22 in the cell nucleus infected with MBV. There was significant difference between them （P〈0.01）. Three type occlusion bodies （OB） were measured. The biggest OB is type Ⅰ and the smallest OB is type Ⅲ The most common OB is type Ⅰ.