辣椒素阻断迷走神经后胃电起搏诱导大鼠脑c-fos表达研究

Expression of c-fos in the brain induced by gastric electrical stimulation in rats after capsaicin application to peripheral vagal fibers

目的以 c-fos 表达为观察指标,探讨迷走神经是否参与外源性胃电起搏调控胃肌电慢波活动。方法将雌性 Wistar 大鼠,分为手术对照组、胃电起搏组、辣椒素对照组和辣椒素胃电起搏组。电起搏组均给予电刺激,以控制慢波为准,持续刺激1 h。用 SP 免疫组化法观察 c-fos 在中枢延髓孤束核(NTS)及迷走神经运动背核(DMV)中的表达。结果胃电起博组 NTS 中 c-fos 阳性细胞数(75.00±9.03)明显高于辣椒素胃电起搏组(19.40±5.50),P<0.01;辣椒素胃电起搏组 NTS 中 c-fos 阳性细胞数与辣椒素对照组(14.00±3.39)和手术对照组(11.80±2.38)相比差异无统计学意义(P>0.05)。胃电起搏组 DMV 中 c-fos 阳性细胞数(35.00±6 28)明显高于辣椒素胃电起博组(13.80±4.21),P<0.01;辣椒素胃电起搏组 DMV 中 c-fos 阳性细胞数与辣椒素对照组(9.20±2.38)和于术对照组(8.20±2.17)相比差异无统计学意义(P>0.05)。结论外源性胃电起搏可调控胃肌电慢波活动,迷走神经可能参与这种调控作用。

Objective To investigate expression of c-fos so that to elucidate if vagus was involved in the neural mechanism that gastric electric stimulation （GES） entrain gastric slow wave. Methods Female Wistar rats were derided into operation control, GES group, capsaicin treatment group and GES group with capsaicin treatment. All rat of GES groups were subjected to GES with parametes which could entrain gastric slow wave. The expression of c-fos in the nucleus of traetus solitarius （NTS） and back movement vagus （DMV） of the central bulb were determined by immunohistoehemieal method. Results The expression of c-fos protein in NTS in GES group was significantly higher than that in GES group with capsaicin treatment （75.00 ± 9.03 vs. 19.40 ±5.50, P 〈 0. 001 ）. There was no significant difference between GES group with capsaicin treatment and eapsaicin treatment group in the expression of c-fos in NTS （ 19.40 ± 5.50 vs. 14.00 ± 3.39, P 〉 0.05 ）. The expression of c-fos protein in DMV in GES group was significantly higher than that in GES group with capsaicin treatment （35.00 ± 6.28 vs. 13.80 ± 4.21 , P 〈 0. 001 ）. There was no significant difference between GES group with capsaiein treatment and capsaicin treatment group in the expression of c-fos in DMV （ 13.80 ±4.21 vs. 9.20 ± 2.38, P 〉 0.05）. Conclusion GES could entrain gastric slow wave, and vagus may be involved in the mechanism underlying the entrainment of gastric myoelectrical activity induced by GES.