多能硫杆菌RubisCO基因（rbcL－rbcS）的克隆及限制性内切酶图谱分析

CLONING OF THE RubisCO GENE(rbcL-rbcS) FROM THIOBACILLUS VERSUTUS AND ANALYSING OF THE RESTRICTION MAP OF THE RECOMBINANT PLASMID

以光合细菌圆球状红杆菌Ｒｈｏｄｏｂａｃｔｅｒｓｐｈａｅｒｏｉｏｄｅｓ的ｒｂｃＬ－ｒｂｃＳ基因为探针，与多能硫杆菌（Ｔｈｉｏｂａｃｉｌｌｕｓｖｅｒｓｕｔｕｓ）染色体ＤＮＡ酶切谱带进行Ｓｏｕｔｈｅｒｎ杂交，检测到了ｒｂｃＬ－ｒｂｃＳ基因的同源序列，又以ｐＵＣ９为载体，克隆了Ｔ．ｖｅｒｓｕｔｕｓ染色体ＤＮＡＰｓｔＩ酶切片段，构建成Ｔ．ｖｅｒｓｕｔｕｓ基因文库，并从这个基因文库中筛选到了含有ＲｕｂｉｓＣＯ基因的重组质粒，将其命名为ｐＳＤＬＳ－１０，进一步对ｐＳＤＬＳ－１０进行了限制性酶切分析。

Rhodobacter sphaeroides rbcL-rbcS DNA was used as a probe to hybridize with Thiobacillus versutus chromosomal DNA digested by restriction enzymes. Hybridization patterns showed that they had homology sequence. We constructed the gene library of T. versutus by using T. versutus PstI fragmemts as donator and pUC9 PstI fragments as vector. The recombinant plasmid, named pSDLS-10, has been isolated from the library and mapped with several restriction endonuclease.