摘要
利用全细胞膜片钳记录技术研究30μg/L和100μg/L缬草单萜氧化物(VMO)对兔单个心室肌细胞L型钙电流(ICaL)和动作电位的影响。结果:30μg/L和100μg/L的VMO使兔心室肌细胞ICaL峰值由6.04±0.59pA/pF分别减至3.99±0.31pA/pF和2.31±0.24pA/pF(n=8,P<0.01);VMO使ICaL的电流电压曲线上移,但不改变其激活电位、电位峰值和反转电位;VMO还使钙电流失活曲线左移。30μg/LVMO可使动作电位时程(APD)明显缩短,APD50和APD90分别缩短了50.3%和29.6%(n=16,P<0.05),而静息电位和动作电位幅值无明显改变。结论:VMO对LCaL具有浓度依赖性阻滞作用。这可能是其对心血管作用的重要机制之一。
Whole cell patch clamp technique was used to investigate the effect of valeriana officinalis L extract（VMO） on L-type calcium current（ICa-L） and action potential duration（APD） in rabbit ventricular myocytes . Results： ①30,100μg/L VMO decreased peak ICa-L（ ICa-Lmax） from 6.04 ± 0.59 pA/pF to 3.99 ± 0. 31 pA/pF and 2.31 ± 0.24 pA/pF（ n = 8,P 〈 0.01 ） ,respectively. VMO upshifted the I-V curves of ICa-L without changes of their active, peak and reverse potentials ;②30μg/L VMO shortened APD50 and APD90 52.2% and 35.6% respectively（ n = 16, P 〈0.05）, but had no significant effect on rest potential and action potential amplitude. Conclusion： VMO blocks ICa-L in a concentration dependent manner and probably inhibits Ica.L in its active state, which may contribute to its cardiovascular effect.
出处
《中国心脏起搏与心电生理杂志》
2005年第6期493-496,共4页
Chinese Journal of Cardiac Pacing and Electrophysiology
