小麦全蚀病菌原生质体制备的条件及再生菌株的致病性

Protoplast Preparation Conditions and Pathogenicity of Regenerated Strains of Gaeumannomyces graminis var.tritici

分别利用崩溃酶、溶壁酶、纤维素酶和蜗牛酶酶解小麦全蚀病菌,进行原生质体的制备试验.结果显示,4种酶均能消化该菌细胞壁,获得一定数量的原生质体;产生原生质体效率最高的是溶壁酶,该酶在浓度为16 mg/mL时产生的原生质体数量最多,最佳的酶解时间为2～3 h,最适作用温度为28℃.制备的原生质体可以再生并与原始出发菌株具有相同的致病能力.

Gaeumannomyces graminis var. tritici strain 9812 was used to prepare protoplast for transformation of the fungus. The protoplast was obtained by digestion of the fungal cell wall with each of the four lyric enzymes, among them lywallzyme had the highest efficiency. Most of the protoplasts were gained with 16 mg/mL lywallzyme for 3 h at 28℃. The prepared protoplasts could regenerate mycelium and produce similar pathogenicity to that of parental strains.