马疫链球菌SH-5生产透明质酸营养条件的研究

Nutritional Condition of Hyaluronic Acid Production by Streptococcus equi HS-5

对Streptococcus equiSH-5生产透明质酸的营养条件进行了研究,摇瓶发酵实验表明该菌株的适宜氮源为酵母膏和牛肉膏以2∶1组成的混合氮源,在碳氮比为2∶1时有利于透明质酸的合成和菌体的生长;通过正交实验摸索出营养培养基的最佳配比为葡萄糖5%,酵母粉6.67%,牛肉膏3.33%,MgSO4.7H2O 0.1%,MnSO4.4H2O 0.02%,NaHCO30.3%,Na2HPO40.5‰,尿嘧啶0.08‰;添加0.05‰异甘草素对产酸有利。

Nutritional conditions of hyaluronic acid prodution by Streptococcus equi HS-5 were studied. The shaking flask experiment indicated that the strain was suitable in 2 ： 1 of yeast extract and beef extract as mixed nitrogen sources, when carbon and nitrogen was at 2： 1, it is conducive to the synthesis of hyaluronic acid production and the bacteria growth. The optimum proportion of the nutritional medium through orthogonal experiment and trying to find out is 5 % glucose, 6.67 % yeast extract, 3.33 % beef extract, 0.1% MgSO4·7H2O, 0.02% MnSO4,·4H2O, 0.3 % NaHCO3, 0.5‰ Na2HPO4, and 0.08‰ uracil. Later it was found that an addition of 0.05‰ of iso-liquiritigenin was conducive to the acid production.