林甸鸡和大骨鸡微卫星DNA标记遗传多样性的比较研究

Comparative Study on Genetic Diversity of Lindian Chicken and Dagu Chicken Breeds Using Microsatellite DNA Markers

为了从分子水平上揭示林甸鸡和大骨鸡的群体遗传结构,利用鸡基因组中5个微卫星标记,检测了林甸鸡和大骨鸡群体的遗传多样性。同时探讨了林甸鸡和大骨鸡在国内几个地方鸡种中的系统地位。结果表明,5个微卫星位点共检测到38个等位基因,其中ADL0146位点的等位基因数5个,为最少,而ADL0136和ADL0185两个位点的等位基因数9个,为最多;5个微卫星位点的平均等位基因数为7.6个。林甸鸡群体内比大骨鸡群体有更丰富的遗传多样性。聚类分析结果显示,林甸鸡和大骨鸡与边鸡、日照麻鸡及寿光鸡之间有比较密切的亲缘关系;而林甸鸡和大骨鸡与鲁西斗鸡的亲缘关系均较远。总体而言,该系统聚类结果与7个鸡种的分化与选育历史是一致的。

For the sake of disclosure of population genetic construction of Lindian chicken and Dagu chicken breeds from molecular level, genetic diversity of Lindian chicken and Dagu chicken was assayed on the basis of 5 microsatellite DNA markers from the chicken gene bank, as well as, the phylogenitic status of Lindian chicken and Dagu chicken was discussed among several native chicken breeds. The results showed, altogether, 38 alleles were detected in 5 microsatellite loci, and among them , the least alleles （5） were detected by ADE0146, and the most alleles （9） were detected by ADL0136 and ADL0185 . The average alleles number was 7.6 from 5 microsatellite loci .Genetic diversity in Lindian chicken was more abundant than that of Dagu chicken. The cluster analysis indicated that the consanguinity was close relatively among Lindian chicken , Dagu chicken, Bian chicken, Rizhao Pockmarked chicken and Shouguang chicken breeds;however, the consanguinity was far relatively between Lindian chicken , Dagu chicken and Luxi fighting chicken breeds. As a while, the clustering diagram reflected well the evolutionary and breeding history of the 7 chicken breeds.