倍他米松对大鼠重症急性胰腺炎肺损伤NF-κB、ICAM-1及IL-6的影响

Effect of Betametasone on NF - κB, IC AM -1 and IL - 6 Following Severe Acute Pancreatitis - associated Lung Injury in Rats

目的探讨重症急性胰腺炎(SAP)相关性肺损伤时,肺组织NF—κB活性、ICAM-1和IL-6水平及倍他米松对三者的影响。方法健康SD大鼠36只分成3组:手术对照组、SAP组、倍他米松组,每组12只。采用5%牛磺胆酸钠(1 mL／kg)诱导复制大鼠SAP模型。倍他米松组在模型复制成功后经静脉泵注倍他米松10 ml/kg。活杀取肺及胰腺,光镜观察组织学病理改变,免疫组织化学法检测肺组织NF—κB活性和ICAM-1水平,ELISA检测大鼠血清和肺组织IL-6水平。结果 (1)病理改变:SAP组胰腺组织和肺组织可见细胞坏死,炎症细胞浸润明显, 微血管充血伴血栓形成等变化。但倍他米松组上述病变程度明显减轻。(2)SAP组NF—κB活性、ICAM-1和IL -6水平显著高于手术对照组和倍他米松组(P<0．01),且倍他米松组NF—κB活性和肺组织IL-6水平高于手术对照组(P<0．05)。结论倍他米松能抑制重症急性胰腺炎相关性肺损伤NF—κB活性和降低ICAM-1和IL-6 水平,减轻重症急性胰腺炎时肺组织损伤。

Objective To study NF -kB activity, ICAM - 1 and IL -6 level following severe acute pancreatitis -associated lung injury and the effect of betametasone on them in rats. Methods 36 SD rats were randomized into three groups each consisting of 12 rats：Sham -operated control group（ C group）, severe acute pancreatitis group （SAP group）, betametasone group （B group）. SAP models were induced by retrograde injection of 5% sodium taurocholate（1 ml/kg） in the pancreatic duct. Group B were injected betametasone 10 ml/kg via vein after SAP models were made successfully. The activity of NF -KB and the level of ICAM -1 in lung were detected by immunohistochemical technique,and the level of I L -6 in serum and lung were measured by ELISA method. Results Histological change：There were some necrosis cells, more neutrophils infiltration, microvascular thrombus in both pancreas and lung tissue in SAP group, but less change in B group. The activity of NF -KB, the level of ICAM -1 and IL -6 in SAP group were significantly higher than those in C and B group（P 〈0.01 ） ,and the activity of NF -KB,the level of IL -6 of lung tissue in B group were higher than in C group （P 〈0.05）. Conclusions Betametasone can inhibit the activity of NF -KB, decrease ICAM -1 and IL -6 level, alleviate severe acute pancreatitis-associated lung injury;animal model.