P43基因在白血病患者弓形虫感染诊断中的应用

Amplification of p43 gene fragement and its application in detection of toxoplasma gondii in leukemia

目的建立快速、特异、敏感诊断白血病患者弓形虫感染情况的DNA检测方法,并分析其临床意义。方法利用ELISA和PCR方法对46例白血病及20名正常人同时进行检测。结果检测白血病40例,PCR和ELISA方法检测弓形虫P43基因片段和其抗原,阳性各为8例及6例,其阳性率分别为17.4%和13.1%。作为阴性对照的20名正常者同时进行弓形虫的检测,其结果均为阴性。结论体外扩增弓形虫P43基因的方法具有准确、快速、特异和敏感的优点,可以作为白血病患者弓形虫感染诊断的有价值的方法之一。

Objective： To establish rapid, specific and sensitive diagnostic technique for the human Toxoplasma gondii infection with leukemia and discuss its clinical significance. Methods：,46 patients of leukemia were detected with ELISA and PCR. Results：Among 46,6 were positive for Toxoplasma gondii antigen,8 were positive based on the PCR. The positive rate were 13.1% and 17.4% in the ELISA and PCR screening respectively. 20 healthy people （anti-Tox antibody negative）were also tested with ELISA and PCR. Conclusion：PCR is an accurate ,relatively rapid ,sensitive and specific method for detecting P43 gene of Toxoplasma gondii,and can be considered a valuable additional tool for identification of Toxoplasma. gondii infections.