HPLC校正因子法测定牛蒡子提取物中总木脂素含量

Quantitative determination of Lignans in Frucyus Arctii's Extracted By corrected factor

目的建立牛蒡子提取物中木脂素含量测定方法,控制牛蒡子提取物有效成分含量。方法采用高效液相色谱法,以C18柱,甲醇-水(1∶1.1)为流动相,以牛蒡苷为对照,用校正因子法测定牛蒡苷、牛蒡苷元、拉帕酚C和拉帕酚H的含量。结果本法重现性好,回收率高,准确度高,牛蒡苷、牛蒡苷元、拉帕酚C和拉帕酚H的含量总和50%以上。结论用本法可不提供牛蒡苷元、拉帕酚C和拉帕酚H含量测定的对照品,只需鉴别和定位用的对照品即可。本法可以基本控制牛蒡子总木脂素的含量,为今后牛蒡子木脂素含量测定提供方便、可行的方法。

Objective： TO establish a HPLC method to determine contents of Lignans in Frucyus Aretii＇ s Extracted, and to control the content of the effective constituents of Frucyus Arctii＇s Extracted. Methods： By means of H.PLC, Using a C18 phase column and methanol- water（1：1.1 ） as mobile phase and using arctiin as contrast, to determine the contents of Aretiin, Aretigenin, Lappaol C and Lappaol H with corrected factor mehod. Results： The method is outstanding in reproducibility,recovery ratio and accuracy. The content of Aretiin, Aretigenin, Lappaol C and Lappaol H reaches 50% and above. Conclusion： Instead of the contracts in determination of contents of Aretiin, Arctigenin, Lappaol C ang Lappaol H, the contracts are required only in identification and immobilization. The medhod is capable on the whole in determination of the content of Lignans in Frucyus Aretii＇ s and will make a good role in quantitative determination of Lignans in Frucyus Aretii＇s in the future researches.