菜青虫体内颗粒体病毒的早期检测

EARLY STAGE DETECTION OF PIERIS RAPAE GRANULOSIS VIRUS IN PIERIS RAPAE LARVAE

用ＥＬＩＳＡ从感染１６ｈ后的菜青虫血淋巴中检出颗粒体病毒，检出率为４８％．检出率随感染时间延长而递增，感染后２４ｈ高达８０％以上，远早于感染病症出现的时间．用血淋巴检测优于组织提取液，阳性滴度高．以１头虫检测较多头虫好．试验证明ＥＬＩＳＡ比对流免疫电泳灵敏．

Pieris rapae granulosis virus in the blood-lymph of Pieris rapae larva can be detected by ELISA after the insect being infected for 16 hours. Detection rate is 48% and it can be reached 80% after 24 hours. Time of detection is much earlier than that of appearance of symptoms. Detecting the blood-lymph is better than doing the liquid drawed from tissue homogenate and detection with a single insect is better than mixture of many insects. The test also proved that ELISA is a lot more sensitive than CIE.