高效液相色谱法测定复方克霉唑软膏中氯霉素和克霉唑的含量

Determination of Chloramphenicol and Clotrimazole Maleate in Compound Clotrimazole by HPLC

目的建立一种快速、准确的高效液相色谱法同时测定复方克霉唑软膏中氯霉素(Chl)和克霉唑(Clo)的含量.方法使用伊利特KromasilC18色谱柱,流动相为甲醇-0.3290mol*L-1磷酸氢二钾(32∶68),检测波长为260nm.结果氯霉素的线性范围为9.96～89.64μg*mL-1,r=0.9992,平均回收率为99.40%;克霉唑的线性范围为63.98～575.82μg*mL-1,r=0.9994,平均回收率为99.79%.结论本方法可快速准确地检测复方克霉唑软膏中氯霉素和克霉唑.

OBJECTIVE A HPLC method for the determination of Chloramphenicol （Chl） and Clotrimazole （Clo） in Compound Clotrimazole was established. METHODS A column （KromasilC18） was used with methanol -0. 3290mol · L^-1KHPO4 （32 ： 68） as mobile phase. RESULTS The detection wave length was 260nm . The linearity range and average recovery rate for Chl were 9.96-89.64μg ~ mL^-1, r =0. 9992, and 99.40% ;while those for Clo were 63.98-575.82μg · mL^-1, r =0.9994, and 99.72% . CONCLUSION The method could be used to detemine the content of Chloramphenicol and Clotrimazole in Compoud Clotrimazole rapidly and accurately.