绿玉树不同来源试管苗抗寒性比较研究

Compared study on cold resistant of plantlets from Euphorbia tirucalli different selection resources

经过对绿玉树不同种源、不同诱变途径选育获得的试管苗进行了与抗寒性相关的形态结构、细胞膜透性,及叶绿体、线粒体超微结构等经低温处理后的变化检测。结果表明,在2℃时,三个种源中(云南、海南、美国引进种)云南种源受冻害最严重。温度降至0℃时,三个种源的绿玉树超微结构变化结果一致:叶绿体、线粒体膨胀成圆球状,并解体。经不同物理化学诱变处理获得试管苗抗寒性都高于未经处理的试管苗,其中以四倍体试管苗抗寒性最高,为获得高抗寒性绿玉树优良无性系奠定了基础。

In order to obtain the high cold-resistant clones of Euphorbia tirucalli, the selections from different provenance and physical-chemical induced mutants were conducted. Morphology, membrane permeability and ultra-structure variations of the selected plantlets under cold-hard- ened were tested. The results indicated that,at 2 ℃ plants from Yunan was the most seriously damaged among of three provenances. However, at 0 ℃ variations of ultra-structures of the three provenance was the same, that both chloroplast and Mitochondria were ball shaped and membranes system were dissolved. Ultra-structure of Tetrapoild was little destroyed compared with ultrastructures of other induced mutant. The resuits suggested that terapoild induced from Colchicin had the highest cold-resistant capacity.